Extracting Onion Dna - 1346 Words

Extraction of DNA from an Onion Molecular biologists and biochemists are involved with research in finding out as much as possible about the DNA in plants. DNA was discovered in the 1950’s, there still remains a lot to be known about it, especially how it is used to determine the physical traits that we all have, and how it regulates the workings of the body. deoxyribonucleic acid is a chemical, we can do reactions with it just like we can work with any other chemical. Experiment: Note: You should write all observations from this lab in the observation section on the third page of this lab. These observations will account for a large part of your grade, so be neat and complete! 1) Prepare a buffer solution by pouring the†¦show more content†¦The (hopefully) clear solution you have in this test tube consists of dissolved DNA fragments, as well as some other biochemical compounds such as RNA and some proteins. DNA is a very long molecule, but compared to the holes in a piece of filtering paper, the molecule is still small enough to pass through. 7) Obtain some ice-cold rubbing alcohol from an ice bath or a freezer. Using a drinking straw, gently add rubbing alcohol to the top until there is about an inch and a half sitting above the buffer solution. The best way to do this is to dip the drinking straw into the isopropanol bottle and then when it has filled to put your finger over the end. To add it to the test tube, slowly let it run down the side of the test tube into the DNA solution. Your goal is to have the rubbing alcohol stay on the top of the DNA solution, with as little mixing as possible. The rubbing alcohol is used to extract the DNA from the onion juice. The reason you want the rubbing alcohol to stay on top of the onion juice is because by doing that the liquid will form two distinct layers. Generally, molecules are attracted to the boundaries of two liquids - sometimes the concentration of large molecules can be much higher at the boundary between two liquids. That’s what we’re hoping for here... if the DNA is attracted to the surface, we can pull most of it out. However, if the alcohol and onion juice mixes too much, there will be too much alcohol throughoutShow MoreRelatedExtraction of DNA from onions1450 Words   |  6 PagesEXTRACTION OF DNA FROM ONIONS ABSTRACT The purpose of the experiment was to experience firsthand the isolation of DNA form a plant tissue without destroying its structure and sequence. A white onion was used for the experiment. After several processes, DNA isolate was the visible result. Different chemical tests were performed on the DNA isolate, namely: Dische Test, Murexide Test, Wheeler-Johnson test and Test for Phosphate. Visible results were then noted. INTRODUCTION DNA (deoxyribonucleicRead MoreShould New Zealand Genetically Modify Crops For Economic Purposes? Essay4171 Words   |  17 PagesGenetic modification has been used in New Zealand since the 1970’s. Genetic modification, also known as Genetic Engineering is the act of directly manipulating the characteristics of an organism by modifying its genetic materials (Deoxyribonucleic acid, DNA) to produce new or improved organisms. In 1996 New Zealand passed the ‘Hazardous Substances and New Organisms’ act which regulates the research and the release of anything that did not already exist in New Zealand. The act includes organisms thatRead MoreCloning Should Not Be Legalized2450 Words   |  10 PagesCloning Should Not be Legalized Human cloning has been a very controversial issue for a number of years. In order to understand cloning better is important to recognize what cloning is. A clone is an exact genetic replica of a DNA molecule, cell, tissue, organ, or entire plant or animal. This may be done through reproductive cloning or therapeutic cloning known as stem cell cloning. (Coalition for the Advancement of Medical Research) Human cloning raises the issues of whether or not a cloned personRead MoreNatural Dyes11205 Words   |  45 Pagesdyes from one-seeded juniper, Juniperus monosperma, Navajo tea, Thelesperma gracile,[28] or alder bark.[29] [edit] Yellows Yellow dyes are about as numerous as red ones,[30] and can be extracted from saffron, pomegranate rind, turmeric, safflower, onion skins, and a number of weedy flowering plants.[30][31] There is limited evidence of the use of weld (Reseda luteola), also called mignonette or dyers rocket [32] before the Iron Age,[30] but it was an important dye of the ancient Mediterranean andRead MoreSexually Transmitted Diseases35655 Words   |  143 PagesPhysical Environment Weather and Climate 100-113 Soils 114-128 Unit 6 Living things in the Environment Plant and animal cells 129 Eukaryotes vs prokaryotes 130 Structures and functions 133-137 Microbes 148-150 Onion and cheek cells 150-151 Ecological study 154 Seed structure and germination 155-157 Reproduction in plants 158-165 Sexually transmitted diseases 166-176 Birth Control and contraceptives 177-185 Effects on population